plasmid addgene Search Results


plenti cmv to neo dest  (Addgene inc)
93
Addgene inc plenti cmv to neo dest
Plenti Cmv To Neo Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiple cloning site mcs  (Addgene inc)
93
Addgene inc multiple cloning site mcs
Multiple Cloning Site Mcs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pma122  (Addgene inc)
93
Addgene inc pma122
Pma122, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid picsl70001∷u6p∷grna  (Addgene inc)
94
Addgene inc plasmid picsl70001∷u6p∷grna
Plasmid Picsl70001∷U6p∷Grna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cre recombination switches expression  (Addgene inc)
93
Addgene inc cre recombination switches expression
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Cre Recombination Switches Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pdest cmv c egfp  (Addgene inc)
93
Addgene inc pdest cmv c egfp
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Pdest Cmv C Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav php eb mdlx nls mruby2  (Addgene inc)
96
Addgene inc aav php eb mdlx nls mruby2
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Aav Php Eb Mdlx Nls Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aavrg hsyn egfp  (Addgene inc)
96
Addgene inc aavrg hsyn egfp
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Aavrg Hsyn Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g pmdlg prre  (Addgene inc)
94
Addgene inc g pmdlg prre
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
G Pmdlg Prre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav1 syn flex jgcamp8f wpre  (Addgene inc)
96
Addgene inc aav1 syn flex jgcamp8f wpre
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Aav1 Syn Flex Jgcamp8f Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p vsvg  (Addgene inc)
96
Addgene inc p vsvg
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
P Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cag cre rbg  (Addgene inc)
93
Addgene inc cag cre rbg
a , <t>Tα1-Cre</t> and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated <t>recombination</t> occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre <t>expression,</t> the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.
Cag Cre Rbg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Tα1-Cre and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated recombination occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre expression, the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.

Journal: bioRxiv

Article Title: Fine-scale excitatory cortical circuits reflect embryonic progenitor pools

doi: 10.1101/363069

Figure Lengend Snippet: a , Tα1-Cre and CβA-FLEx plasmids were delivered by IUE at different embryonic ages (E12.5 to E15.5). Quantification of GFP + and TdTomato + cells in the VZ, 24 h after each time point for IUE, revealed that a significant proportion of labelled cells were GFP + . This is consistent with the idea that Tα1 + progenitors represent a significant progenitor population in the embryonic mouse VZ. Error bars indicate standard error of the mean. b , Quantifying GFP + cells at different time points (2 d, 3 d, >21 d) following IUE at E14.5, revealed that the proportion of SNP-derived cells remains relatively stable, consistent with the idea that the majority of Cre-mediated recombination occurs within 24 h of IUE. c , Quantification of GFP + and TdTomato + cells in the VZ, 24 h after IUE with different ratios of Tα1-Cre to CβA-FLEx plasmid. Consistent with the idea that labelling accurately reflects the promoter driving Cre expression, the proportion of GFP + and TdTomato + VZ cells was stable across a range of plasmid ratios. A plasmid ratio of 1:1 was used for electrophysiological studies.

Article Snippet: Plasmid DNA included: (i) ‘Tα1-Cre’, in which the gene for Cre recombinase is under the control of a portion of the Tα1 promoter ; (ii) ‘CβA-FLEx’ which uses the chicken β-actin promoter to control a flexible excision (FLEx) cassette in which Cre recombination switches expression from TdTomato fluorescent protein to enhanced green fluorescent protein ; (iii) ‘DIO-ChR2-mCherry’ (pAAV-EF1a-doublefloxed-hChR2(H134R)-mCherry-WPRE-HGHpA; Addgene #20297), in which Cre recombination turns on the expression of channelrhodopsin-2 (ChR2) under the control of the human elongation factor-1a promoter ; (iv) DO-ChR2-mCherry (‘Cre-Off’; pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA; Addgene #37082 in which Cre recombination turns off the expression of ChR2 under the control of the human elongation factor-1a promoter ; and (v) DIO-ChR2-EYFP (pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA; Addgene #20298), which is equivalent to DIO-ChR2-mCherry, except that EYFP replaces mCherry.

Techniques: Derivative Assay, Plasmid Preparation, Expressing